外泌体研究
外泌体研究服务介绍
外泌体(exosomes)是一种能被机体内大多数细胞分泌的直径大约为 30 ~ 150 nm 的具有脂质双分子层膜结构的微小囊泡。大多数类型的细胞都可以分泌外泌体,同时外泌体也广泛存在于体液中,包括血液、眼泪、尿液、唾液、乳汁、腹水等。目前研究发现外泌体中富含核酸(mRNA、microRNA、lncRNA、circRNA 等)、蛋白、脂质等活性生物分子。外泌体频繁穿梭于细胞之间,为细胞之间的通信提供了桥梁。因此,外泌体几乎可以与任何疾病相关联,成为疾病标志物、疾病机理、药物开发等研究的创新热点。
| 华盈生物外泌体服务体系
外泌体的研究是一项系统工程,从生物样本的保存开始,到外泌体的提取、鉴定与质检、内容物组学分析、验证、功能实验等,均需较高技术水准作为保障才能够确保实验结果的优质可靠。华盈生物自2017 年始,全力打造外泌体“一站式”研究服务平台,逐步建立了包含外泌体的提取及鉴定、外泌体标记/ 示踪、外泌体蛋白质组学/转录组学分析等全方位技术体系,以“高品质服务”为宗旨,力求为广大科研用户提供在外泌体研究方面全方位的支持和便利。
| 华盈生物外泌体服务优势
华盈生物根据不同的样品类型和研究目的,推出优选的外泌体分离方法,并提供全套的外泌体研究技术服务。在分离高质量外泌体的基础上,进行外泌体中的蛋白质和核酸组分的高通量筛选和分析。华盈生物开发的组学研究技术服务体系进行了系统性创新优化,将样本用量降低的同时获取高质量数据。华盈生物利用1mL血浆即可进行外泌体的分离和测序,并能稳定获得超过1万条长链RNA。同时,华盈生物利用0.5mL血浆即可完成外泌体的分离与蛋白质组筛选工作,并不断冲击外泌体蛋白质组学检测的上限,例如:在人外泌体血浆蛋白组鉴定历史记录已达到2336种蛋白质,并且这个数据记录每天都可能被再次突破。
| 华盈生物外泌体项目经验
截止目前,华盈生物已经完成超过30种样品类型的样品外泌体制备与分析。对于特殊样本如:胃液、脑积液、脑脊液、胆汁、微生物培养液、植物培养液等,也累积了丰富的项目经验,可承接复杂样品和项目的研究服务需求。
| 客户文献
[1]. Chen R, Feng T, Cheng S et al. Evaluating the defect targeting effects and osteogenesis promoting capacity of exosomes from 2D-and 3D-cultured human adipose-derived stem cells. Nano Today, 2023, 49: 101789.(中国人民解放军总医院)IF=17.4
[2]. Du X, Jiang Y, Zhang Q et al. Genome-Wide Profiling of Exosomal Long Noncoding RNAs Following Air Pollution Exposure: A Randomized, Crossover Trial. Environ Sci Technol. 2023 Feb 21;57(7):2856-2863.(复旦大学公共卫生学院)IF=11.4
[3]. Luo Liwen, Gong Junfeng, Wang Zhouguang, et al. Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. Bioact Mater, 2022, 15: 29-43. (第三军医大学新桥医院)IF=18.9
[4]. Zhang Luwen, Zhao Qiong, Cang Hui et al. Acute Myeloid Leukemia Cells Educate Mesenchymal Stromal Cells toward an Adipogenic Differentiation Propensity with Leukemia Promotion Capabilities. Adv Sci (Weinh), 2022, undefined: e2105811. (浙江大学医学院附属儿童医院)IF=15.1
[5]. Du Xihao, Zhang Qingli, Jiang Yixuan, et al. Dynamic molecular choreography induced by traffic exposure: A randomized, crossover trial using multi-omics profiling. J Hazard Mater, 2022, 424: 127359.(复旦大学公共卫生学院)IF=13.6
[6]. Xiang Z, Jiang B, Li W et al. The diagnostic and prognostic value of serum exosome-derived carbamoyl phosphate synthase 1 in HEV-related acute liver failure patients. J Med Virol. 2022 Oct;94(10):5015-5025.(姑苏医学院附属苏州医院)IF=12.7
[7]. Du X, Zhang Q, Jiang Y et al. Characterization of plasma-derived exosomal miRNA changes following traffic-related air pollution exposure: A randomized, crossover trial based on small RNA sequencing. Environ Int. 2022 Sep;167:107430.(复旦大学公共卫生学院)IF=11.8
[8]. Qiao H, Mei J, Yuan K et al. Immune-regulating strategy against rheumatoid arthritis by inducing tolerogenic dendritic cells with modified zinc peroxide nanoparticles. J Nanobiotechnology. 2022 Jul 14;20(1):323.(上海市第九人民医院)IF=10.2
[9]. Xia Chengjie, Xu Weiming, Ai Xin, et al. Autophagy and Exosome Coordinately Enhance Macrophage M1 Polarization and Recruitment in Influenza A Virus Infection. Front Immunol, 2022, 13: 722053. (复旦大学药学院)IF=7.3
[10]. Feng W, Jin Q, Ming-Yu Y et al. MiR-6924-5p-rich exosomes derived from genetically modified Scleraxis-overexpressing PDGFRα(+) BMMSCs as novel nanotherapeutics for treating osteolysis during tendon-bone healing and improving healing strength. Biomaterials. 2021 Dec;279:121242.(第三军医大学第一附属医院)IF=14.0
[11]. Zhang Z, Xu R, Yang Y et al. Micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and secretion to improve osseointegration. J Nanobiotechnology. 2021 Mar 19;19(1):78.(中山大学附属口腔医院)IF=10.2
[12]. Qiao Yang, Mingjing Chen, Jiaoyang Gu, et al. Novel Biomarkers of Dynamic Blood PD-L1 Expression for Immune Checkpoint Inhibitors in Advanced Non-Small-Cell Lung Cancer Patients. Front Immunol. 2021 Apr 16;12:665133.(陆军军医大学新桥医院)IF=7.3
[13]. Wang T, Jian Z, Baskys A, et al. MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system. Biomaterials, 2020, 257: 120264. (西安市中心医院)IF=14.0
[14]. Chen CW, Fu M, Du ZH et al. Long Noncoding RNA MRPL23-AS1 Promotes Adenoid Cystic Carcinoma Lung Metastasis. Cancer Res. 2020 Jun 1;80(11):2273-2285.(北京大学口腔医学院和口腔医院中心实验室)IF=11.2
[15]. Qiao L, Hu S, Liu S et al. microRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential. J Clin Invest. 2019 Apr 29;129(6):2237-2250.(北卡罗来纳州立大学分子生物医学科学系)IF=15.9
[16]. Ruixiang Li, Yuwei He, Ying Zhu, et al. Expressway to Rheumatoid Arthritis by Macrophage-derived Microvesicle-Coated Nanoparticles. Nano Lett. 2019 Jan 9;19(1):124-134.(复旦大学智能化递药教育部重点实验室)IF=10.8
[17]. Zhenzhen Chen, Liangshun You, Lei Wang, et al. Dual effect of DLBCL-derived EXOs in lymphoma to improve DC vaccine efficacy in vitro while favor tumorgenesis in vivo. J Exp Clin Cancer Res. 2018 Aug 13;37(1):190.(浙江大学医学院附属第一医院)IF=11.3
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