单通路磷酸化抗体芯片

      从细胞膜、胞浆到细胞核，存在多条信号通路串联交叉形成的复杂信号网络。该信号网络在细胞受到胞外刺激后将信号通过级联放大、分散调节等方式传入胞内，引起一系列的综合性细胞应答。一种生物效应的出现往往存在多条信号通路的同步活化，可逆的磷酸化修饰反应则是细胞内部最为普遍和节能的信号蛋白活化调节方式。因此，找到激活的信号通路乃至发生磷酸化调变的通路蛋白，往往成生命科学研究的起点。

 

 

 

 

 

       信号通路磷酸化抗体芯片，采用三维高分子膜专用技术，在片基上高密度结合上百种高特异抗体，一次性可对特定经典信号通路上的信号蛋白进行全面筛选和系统分析。芯片针对每一个特定蛋白磷酸化位点，设置一对抗体分别检测其磷酸化（Phospho）和非磷酸化（non-Phospho）状态以提高磷酸化检测灵敏度和稳定性。由于信号蛋白会有多个重要磷酸化位点，这些磷酸化位点的功能各异。芯片设计时，针对同一个蛋白的不同磷酸化位点分别采用不同的配对抗体进行检测，尽可能做到全面、高效，是信号通路系统研究必不可少的工具。

|   抗体芯片特点

●  芯片规格为76 x 25 x 1 mm；

●  每种抗体设置6次技术重复；

●  适用于组织、细胞等多类型样本；

●  5 x10⁶细胞、100μg总蛋白量即可满足实验；

●  每个检测位点设有磷酸化和非磷酸化配对抗体；

●  可通用于人、小鼠、大鼠等多类型模式生物检测。

|   芯片产品列表（点击产品名称，下载抗体列表）

|   抗体芯片原理

|   服务资质

       上海华盈生物医药科技有限公司（华盈生物）推出的信号通路磷酸化抗体芯片产品，由美国Full Moon公司研发和生产。华盈生物技术团队与美国Full Moon公司自2009年开始即达成战略合作关系，在中国市场共同推广磷酸化抗体芯片技术。

       华盈生物秉持专业化的服务理念，着力打造中国专业蛋白芯片/抗体芯片服务的品牌标杆。华盈生物以高品质的数据质量为服务导向，在售前支持、样品管理、实验操作、数据分析、售后解答等各服务环节实行ISO9001质量管理体系一体化管理，确保科研合作者在获取高标准芯片数据的同时，也能收获优质的服务体验。

|   客户文献

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