去外泌体血清（无外泌体血清）

产品名称：去外泌体胎牛血清（Exo-FBS）

英文名称：Exosome depleted Fetal Bovine Serum

品牌：ViVaCell

货号：C38010050

规格：50 mL/瓶

贮存：-20℃

说明：该产品仅供科研使用

 

 

 

      胎牛血清（Fetal Bovine Serum, FBS）是大多数细胞培养重要的生长支持物。天然来源的FBS会含有大量的外泌体（exosome）等囊泡（Extracellular Vesicles）成分。由于FBS来源的外泌体会对研究结果产生巨大干扰，因此，外泌体研究时必须使用去除外泌体后的FBS进行细胞培养。

|   产品特点

1. 专为外泌体研究而设计；

2. 无菌采集的健康胎牛血清过滤法去除外泌体；

3. 胎牛血清内源外泌体去除率达97%；

4. 过滤法可有效保护FBS中重要的细胞营养因子；

5. Exo-FBS培养的细胞生长速率与形态与普通FBS无差异。

|   产品质控

（FBS：常规胎牛血清，Exo-FBS：去外泌体血清）

  图1 A549细胞在常规FBS和Exo-FBS中生长情况一致

 图2 Western blot结果显示Exo-FBS中TSG101含量显著降低

|   产品说明

       细胞在含常规FBS培养基中培养一定时间，细胞融合度约为60% - 70%时，移去原有含FBS的培养基，换成新鲜的含无外泌体血清的培养基，继续培养48h左右，细胞融合度达到80%-95%左右时，收取上清用于提取外泌体。

|   客户文献

[1]. Hu Y, Wu B, Xiong Y, et al. Cryogenic 3D printed hydrogel scaffolds loading exosomes accelerate diabetic wound healing. Chemical Engineering Journal. 2021；426:130634. IF=13.2

[2]. Yu L, Sui B, Fan W, et al. Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p. J Extracell Vesicles. 2021 Jan;10(3):e12056. IF=25.8

[3]. Hu Y, Tao R, Chen L, et al. Exosomes derived from pioglitazone-pretreated MSCs accelerate diabetic wound healing through enhancing angiogenesis. J Nanobiotechnology. 2021 May 21;19(1):150. IF=10.4

[4]. Deng Z, Wang J, Xiao Y, et al. Ultrasound-mediated augmented exosome release from astrocytes alleviates amyloid-β-induced neurotoxicity. Theranostics. 2021 Feb 25;11(9):4351-4362. IF=8.5

[5]. Liu S, Chen J, Shi J, et al. M1-like macrophage-derived exosomes suppress angiogenesis and exacerbate cardiac dysfunction in a myocardial infarction microenvironment. Basic Res Cardiol. 2020 Feb 28;115(2):22. IF=17.1

[6]. Qiao S, Zhang W, Yin Y, et al. Extracellular vesicles derived from Krüppel-Like Factor 2-overexpressing endothelial cells attenuate myocardial ischemia-reperfusion injury by preventing Ly6Chigh monocyte recruitment. Theranostics. 2020 Sep 18;10(25):11562-11579. IF=11.5

[7]. Wang Y, Lu J, Chen L, et al. Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC. Mol Ther Nucleic Acids. 2020 Jun 5;20:421-437. IF=8.8

[8]. Gao X, Xiong Y, Li Q, et al. Extracellular vesicle-mediated transfer of miR-21-5p from mesenchymal stromal cells to neurons alleviates early brain injury to improve cognitive function via the PTEN/Akt pathway after subarachnoid hemorrhage. Cell Death Dis. 2020 May 13;11(5):363. IF=8.4