蛋白质组学详细

糖基化蛋白质组学 > 凝集素富集糖基化蛋白质组学

    凝集素富集糖基化蛋白质组学

     

            凝集素(Lectin)对于特定的糖结构具有结合特异性。将蛋白质酶解后,先利用Lectin富集N-糖基化肽段,然后用N-糖酰胺酶(PNGase)在H218O中切除连接在天冬酰胺残基(Asn)上的糖链,最后通过液相色谱质谱(LC-MS)鉴定脱糖后的多肽,即可获得差异糖链背后的多肽、蛋白及糖苷键位置信息。

            华盈生物拥有芯片质谱双平台优势,创新性地将Lectin Array与LC-MS进行结合,提供从差异Lectin芯片筛选、糖蛋白的Lectin富集,到糖蛋白LC-MS鉴定一整套系统性的糖基化蛋白组研究方案。

     

     

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    技术路线



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    样品要求



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    应用方向


                         ◇  
    糖蛋白疾病标志物研究

                         ◇  新蛋白质修饰功能研究

                         ◇  新型蛋白质分子调控机制研究

                         ◇  特定蛋白表面糖谱构建及差异检测

     

    凝集素芯片指标

     

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    客户代表文章


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