蛋白芯片详细

信号通路磷酸化抗体芯片 > 肿瘤信号通路磷酸化广谱筛选抗体芯片(CSP100)

肿瘤信号通路磷酸化广谱筛选抗体芯片(CSP100)


肿瘤,在增殖、分化、凋亡、侵袭、炎症等方面具有复杂的生物学行为,是目前最难攻克的疾病之一。信号通路在肿瘤细胞自身生命活动,以及与基质细胞信号交往中起着关键作用,而每种胞行为的改变都存在多条信号通路的共同参与。层出不穷的抗癌药物耐受现象表明,单一信号通路的调节方式不能从根本上解决肿瘤治疗的问题。对肿瘤相关信号通路的整体把握和筛选,也因此成为肿瘤相关科学研究的起点。

        肿瘤信号通路磷酸化广谱筛选抗体芯片(CSP100),采用三维高分子膜专利技术,在片基上共价结合269种高特异抗体,分别检测93个信号蛋白的132个磷酸化位点。这些信号蛋白广泛参与多条与肿瘤相关信号通路的传导过程。芯片针对每一个特定蛋白磷酸化位点,设置一对抗体分别检测其磷酸化(Phospho)和非磷酸化(non-Phospho)状态,以提高磷酸化检测灵敏度和稳定性。一次芯片实验即可实现多条信号通路的同步筛选和关键调变位点的清晰定位,在肿瘤的发生、发展、转移、耐药、复发、微环境等机制研究发挥重要作用。同时,由于疾病信号通路的共性特点,CSP100也成为其它疾病研究中信号通路筛选的重要工具。

                                        

抗体芯片特点:

 

  • 芯片规格为76 x 25 x 1 mm;

  • 实现12条信号通路全面筛选;

  • 每种抗体设置6次技术重复;

  • 适用于组织、细胞等多类型样本;

  • 5 x106细胞、200μg总蛋白量即可满足实验;

  • 每个检测位点设有磷酸化和非磷酸化配对抗体;

  • 可通用于人、小鼠、大鼠等多类型模式生物检测。

           

芯片检测列表:

信号通路

蛋白数

信号通路

蛋白数

MAPK signaling pathway

28

Regulation of actin cytoskeleton

15

Apoptosis signaling pathway

23

Wnt signaling pathway

14

AKT signaling pathway

22

VEGF signaling pathway

14

Cell cycle

18

NF-KB signaling pathway

13

Jak-STAT signaling pathway

17

mTOR signaling pathway

10

p53 signaling pathway

16

TGF-beta signaling pathway

9



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抗体芯片原理:

                                    

                                                       

                                        

抗体芯片文献:

1.      Huang N, et al. Effect of Splenectomy on Serum Cytokine Profiles in Hepatitis B Virus-Related Cirrhosis Patientswith Portal Hypertension. Viral Immunol, 2018: 1-8. 西安交通大学附属第二医院)
2.       Liu D, et al. C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. Nat Commun. 2018, 9(1): 1739. (华中科技大学同济医院)
3.      Lei miao Yin, et al. Transgelin-2 as a therapeutic target for asthmatic pulmonary resistanceSci Transl Med,2018, 10(427).(上海中医药大学)
4.      Venkatesh, Humsa S., et al. Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.Nature, 2017, 549(7673): 533-537. 
5.      Gao Y, et al. Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis.Natstruct Mol Biol, 2016, 23(5): 441-449. (中国科学院生物物理研究所)

6.     Jiang HL et al. SSRP1 uppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde Signaling, Cancer Res, 2016, 76(4): 952-64.(复旦大学附属肿瘤医院)

7.      Kuang XY et al. The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasis, Cancer Lett, 2016, 377(1): 87-96.复旦大学附属肿瘤医院)

8.      Chen Y, et al. The hepatitis B virus X protein promotes pancreatic cancer through modulation of the PI3K/AKT signaling pathwayCancer Lett, 2016, 380(1): 98-105. (浙大第二附属医院)

9.       Zhu Y et al. Long non-coding RNA LOC572558 inhibits bladder cancer cell proliferation and tumor growth by regulating the AKT-MDM2-p53 signaling axisCancer Lett, 2016, 04(3).(复旦大学附属肿瘤医院)

10.        Luo L L, Zhao L, Wang Y X, et al. Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5: 17336.  (中山大学附属肿瘤医院)

11.       Zhu Y P, Wan F N, Shen Y J, et al.  Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.(复旦大学附属肿瘤医院)
12.      Wang T, Han S, Wu Z, et al.  XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer. Biochem Bioph Res Co, 2015, 464(2): 635-641. (上海长征医院)

13.      Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt-p27(Kip1) axis. Mol Carcinogen, 2015, 54(12): 1678-85. (福建医科大学附属协和医院)

14.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (复旦大学附属肿瘤医院)

15.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater, 2016, 29: 320-32.  (同济大学)

16.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015, 55(11): 1739-1746. (河北大学)

17.      Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis,2014, 35(7): 1573-1581.(上海交通大学医学院)

18.      Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res, 2014, (24): 204-217. (上海仁济医院) 

19.      Xu N et al. Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-kB and JAK-STAT kinase signaling pathways. Int J Mol Med, 2013, 32(3): 729-735.(吉林大学)

20.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013, 8(4): e61195.(第三军医大学)

21.      Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen. 2012, 20(5): 778-785.

22.       Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death Dis, 2012, 3: e406.(西安交通大学)