蛋白芯片详细

信号通路磷酸化抗体芯片 > 信号通路磷酸化广谱筛选抗体芯片(PEX100)

信号通路磷酸化广谱筛选抗体芯片(PEX100)

       从细胞膜、胞浆到细胞核,存在多条信号通路串联交叉形成的复杂信号网络。该信号网络在细胞受到胞外刺激后将信号通过级联放大、分散调节等方式传入胞内,引起一系列的综合性细胞应答。一种生物效应的出现往往存在多条信号通路的同步活化,可逆的磷酸化修饰反应则是细胞内部最为普遍和节能的信号蛋白活化调节方式。因此,找到激活的信号通路乃至发生磷酸化调变的通路蛋白,往往成生命科学研究的起点。  

                                                                               

     
        信号通路磷酸化广谱筛选抗体芯片(PEX100),采用三维高分子膜专利技术,在片基上高密度结合  1318  种高特异抗体,分别检测432个信号蛋白的679个磷酸化位点。这些信号蛋白广泛参与多条重要信号通路信号传导过程。芯片针对每一个特定蛋白磷酸化位点,设置一对抗体分别检测其磷酸化(Phospho)和非磷酸化(non-Phospho)状态以提高磷酸化检测灵敏度和稳定性。一次芯片实验即可实现多条信号通路的同步筛选和具体调变位点的清晰定位,为后续生物现象的深入探索提供明确的研究方向。

                                                                                                    

抗体芯片特点:


  • 芯片规格为76 x 25 x 1 mm;

  • 实现31条信号通路全面筛选;

  • 每种抗体设置2次技术重复;

  • 适用于组织、细胞等多类型样本;

  • 5 x106细胞、200μg总蛋白量即可满足实验;

  • 每个检测位点设有磷酸化和非磷酸化配对抗体;

  • 可通用于人、小鼠、大鼠等多类型模式生物检测。

           

 

芯片检测范围:

信号通路蛋白数信号通路蛋白数

MAPK signaling pathway

67

Regulation of actin cytoskeleton

36

Erk signaling pathway

59

Jak-STAT signaling pathway

32

Angiogenesis

57

AKT/PKB signaling pathway

31

Focal adhesion

56

FGF signaling pathway

29

Neurotrophin signling pathway

52

Endocytosis

29

ErbB signaling pathway

45

Integrin signaling pathway

29

AKT signaling pathway

45

VEGF signaling pathway

27

Chemokine signaling pathway

45

Wnt signaling pathway

26

PDGF signaling pathway

43

p53 signaling pathway

25

T cell receptor signaling pathway

43

Adipocytokine signaling pathway

19

Apoptosis signaling pathway

41

Calcium signaling pathway

19

EGF receptor signaling pathway

41

Insulin/IGF pathway

16

Insulin signaling pathway

39

mTOR signaling pathway

16

CREB signaling pathway

39

TGF-beta signaling pathway

13

Cell cycle

37

Interleukin signaling pathway

37


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抗体芯片原理:


                                                                                                  

                 

                                                                                                    

抗体芯片文献:


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14.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (复旦大学附属肿瘤医院)

15.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater, 2016, 29: 320-32.  (同济大学)

16.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015, 55(11): 1739-1746. (河北大学)

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20.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013, 8(4): e61195.(第三军医大学)

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