蛋白芯片详细

信号通路磷酸化抗体芯片 > Cytoskeleton细胞骨架信号通路磷酸化抗体芯片(PCP141)

Cytoskeleton细胞骨架信号通路磷酸化抗体芯片(PCP141)

       Cytoskeleton 是一种在真核和原核生物的细胞质中均广泛存在的动态三维蛋白质结构,分为Actin Filaments、Intermediate filaments及Microtubules三种蛋白丝类型。Cytoskeleton扮演着肌肉和骨架双重角色,在细胞保护、迁移、分裂、胞内物质转运及细胞器稳定方面均发挥重要作用。Cytoskeleton接收细胞表面GPCRs(G Protein-Coupled Receptors)、RTKs(Receptor Tyrosine Kinases)、整合素及其它特定受体传递的信号,引发细胞形态、迁移、增殖和生存状态的改变。一旦Cytoskeleton信号通路发生紊乱,胞外刺激与细胞活动就会发生脱节,就会引发免疫障碍、肿瘤等多种疾病的发生。

        Cytoskeleton 细胞骨架信号通路磷酸化抗体芯片(PCP141,采用三维高分子膜专利技术,在抗体芯片片基上共价结合141种高特异抗体,并运用特有的荧光标记技术进行样本标记,以实现对Cytoskeleton经典信号通路的高覆盖检测。抗体芯片提供信号蛋白多个关键磷酸化位点的同步检测,针对每一个特定蛋白磷酸化位点,设置一对抗体分别检测其磷酸化(Phospho)和非磷酸化(non-Phospho)状态。同时,该抗体芯片可检测多种已有文献报道的非Cytoskeleton经典通路的信号蛋白,极大扩展信号通路芯片研究的延伸性。


抗体芯片特点:

           

1. 芯片规格为76 x 25 x 1 mm;                

2. 实现单一信号通路全面筛选;                 

3. 每种抗体设置6次技术重复;                 

4. 适用于组织、细胞等多类型样本;                

5. 5x106细胞、200μg总蛋白量即可满足实验;              

6. 每个检测位点设有磷酸化和非磷酸化配对抗体;                

7. 可通用于人、小鼠、大鼠等多类型模式生物检测。                


 

抗体芯片原理:

                                                       

                                        

 

抗体芯片列表:


 

                                                                                                                                  
   动态箭头.gif详细列表下载

                   


抗体芯片文献:  


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5.     Jiang HL et al. SSRP1 uppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde Signaling, Cancer Res, 2016, 76(4): 952-64.(复旦大学附属肿瘤医院)

6.      Kuang XY et al. The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasis, Cancer Lett, 2016, 377(1): 87-96.复旦大学附属肿瘤医院)

7.      Chen Y, et al. The hepatitis B virus X protein promotes pancreatic cancer through modulation of the PI3K/AKT signaling pathwayCancer Lett, 2016, 380(1): 98-105. (浙大第二附属医院)

8.       Zhu Y et al. Long non-coding RNA LOC572558 inhibits bladder cancer cell proliferation and tumor growth by regulating the AKT-MDM2-p53 signaling axisCancer Lett, 2016, 04(3).(复旦大学附属肿瘤医院)

9.        Luo L L, Zhao L, Wang Y X, et al. Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5: 17336.  (中山大学附属肿瘤医院)

10.       Zhu Y P, Wan F N, Shen Y J, et al.  Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.(复旦大学附属肿瘤医院)
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12.      Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt-p27(Kip1) axis. Mol Carcinogen, 2015, 54(12): 1678-85. (福建医科大学附属协和医院)

13.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (复旦大学附属肿瘤医院)

14.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater, 2016, 29: 320-32.  (同济大学)

15.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015, 55(11): 1739-1746. (河北大学)

16.      Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis,2014, 35(7): 1573-1581.(上海交通大学医学院)

17.      Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res, 2014, (24): 204-217. (上海仁济医院) 

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19.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013, 8(4): e61195.(第三军医大学)

20.      Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen. 2012, 20(5): 778-785.

21.       Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death Dis, 2012, 3: e406.(西安交通大学)