蛋白芯片详细

信号通路磷酸化抗体芯片 > AMPK信号通路磷酸化抗体芯片(PAM174)

AMPK信号通路磷酸化抗体芯片(PAM174)

 

      
        Ser/Thr蛋白激酶AMPK(Adenosine 5'-Monophosphate (AMP)-activated Protein Kinase)是生物能量代谢调节的关键分子。AMPK在诸如低糖、缺氧、缺血、休克等引起的细胞内ATP急速减少的情况下被激活,进而通过调节其下游的转录因子、酶等磷酸化水平来抑制糖原、脂类及蛋白质等的生物合成过程。AMPK与mTOR(Mammalian Target of Rapamycin)等信号通路密切相关,在细胞自噬、极化等生命活动中有突出作用。其与2型糖尿病、肥胖、衰老、肿瘤等疾病的关系日益明确。


        AMPK 信号通路磷酸化抗体芯片(PAM174),采用三维高分子膜专利技术,在抗体芯片片基上共价结合174种高特异抗体,并运用特有的荧光标记技术进行样本标记,以实现对AMPK经典信号通路的高覆盖检测。抗体芯片提供信号蛋白多个关键磷酸化位点的同步检测,针对每一个特定蛋白磷酸化位点,设置一对抗体分别检测其磷酸化(Phospho)和非磷酸化(non-Phospho)状态。同时,该抗体芯片可检测多种已有文献报道的非AMPK经典通路的信号蛋白,极大扩展AMPK单一信号通路研究的延伸性。

 

抗体芯片特点

 

1. 芯片规格为76 x 25 x 1 mm;                

2. 实现单一信号通路全面筛选;                 

3. 每种抗体设置6次技术重复;                 

4. 适用于组织、细胞等多类型样本;                

5. 5x106细胞、200μg总蛋白量即可满足实验;              

6. 每个检测位点设有磷酸化和非磷酸化配对抗体;                

7. 可通用于人、小鼠、大鼠等多类型模式生物检测。                

                                        

抗体芯片原理

 


                 

                 

抗体芯片列表


  动态箭头.gif详细列表下载

抗体芯片文献:


1.      Lei miao Yin, et al. Transgelin-2 as a therapeutic target for asthmatic pulmonary resistanceSCI TRANSL MED,2018.(上海中医药大学)
2.      Venkatesh, Humsa S., et al. Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.Nature 549.7673 (2017): 533.
3.      Gao Y, et al. Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis.Natstruct Mol Biol, 2016, 23(5): 441-449. (中国科学院生物物理研究所)

4.     Jiang HL et al. SSRP1 uppresses TGF-β-Driven Epithelial-to-Mesenchymal Transition and Metastasis in Triple-Negative Breast Cancer by Regulating Mitochondrial Retrograde Signaling, Cancer Res, 2016, 76(4): 952-64.(复旦大学附属肿瘤医院)

5.      Kuang XY et al. The phosphorylation-specific association of STMN1 with GRP78 promotes breast cancer metastasis, Cancer Lett, 2016, 377(1): 87-96.(复旦大学附属肿瘤医院)

6.      Chen Y, et al. The hepatitis B virus X protein promotes pancreatic cancer through modulation of the PI3K/AKT signaling pathwayCancer Lett, 2016, 380(1): 98-105. (浙大第二附属医院)

7.       Zhu Y et al. Long non-coding RNA LOC572558 inhibits bladder cancer cell proliferation and tumor growth by regulating the AKT-MDM2-p53 signaling axisCancer Lett, 2016, 04(3).(复旦大学附属肿瘤医院)

8.        Luo L L, Zhao L, Wang Y X, et al. Insulin-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5.  (中山大学附属肿瘤医院)

9.       Zhu Y P, Wan F N, Shen Y J, et al.  Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.(复旦大学附属肿瘤医院)
10.      Wang T, Han S, Wu Z, et al.  XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer. Biochem Bioph Res Co, 2015, 464(2): 635-641. (上海长征医院)

11.      Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti‐CD44 therapy partly through regulating PI3K/Akt–p27Kip1 axis. Mol Carcinogen, 2015, 54(12): 1678-85. (福建医科大学附属协和医院)

12.      Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10. (复旦大学附属肿瘤医院)

13.      Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater, 2016, 29: 320-32.  (同济大学)

14.      Li W, et al.  Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model. Mol Carcinog, 2015. (河北大学)

15.      Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis April 7, 2014.上海交通大学医学院

16.      Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res, 2014, (24): 204-217. (上海仁济医院) 

17.      Xu N et al. Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-κB and JAK-STAT kinase signaling pathways. Int J Mol Med, 2013, 32(3): 729-735.(吉林大学)

18.      Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013, 8(4): e61195.(第三军医大学)

19.      Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen. 2012, 20(5): 778-85.

20.       Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death Dis, 2012, 3: e406.(西安交通大学)