蛋白芯片详细

蛋白质组芯片 > 凝集素蛋白芯片 > 凝集素蛋白芯片

    凝集素蛋白芯片

            凝集素(Lectin)是一类自然界广泛存在的蛋白质,具有特定糖结构识别的特性。糖蛋白和糖脂上发生的糖基化修饰,在细胞分化、病原微生物侵袭,以及肿瘤的发生、发展和迁移等生物现象中扮演着极为重要的角色。利用凝集素进行特定糖结构的解析是糖生物学研究领域的常用手段。本芯片将可商业获得的56种经典凝集素,以微阵列的形式固定在高分子三维芯片片基上,形成高通量凝集素芯片,可进行多种糖结构的同步筛选和交叉验证。该芯片的诞生为专注于分析细胞表面糖基化及蛋白质糖基化研究的科学家提供了极佳的工具。

     

    Sharon N,et al.Glycobiology. 2004

     

    蛋白芯片特点:

    1. 凝集素种类:56种 

    2. 可检测样品:12个

    3. 技术重复:3次

    4. 点样环境:温度4-8℃,湿度30%~40%

    5. 芯片基片:高分子三维基片,尺寸为75*25 mm

    6. 点直径:~120微米

    7. 点间距:X向270微米,Y向300微米(圆心距)

    8. 点体积:0.5~1 nL/点

    9. 芯片点制后处理:4℃过夜固定后-80℃长期保存

    蛋白芯片应用:

    1. 特定蛋白表面糖谱构建及差异检测;

    2. 活细胞表面糖谱构建及差异检测;

    3. 细胞或组织裂解液糖谱构建及差异检测 ;

    4. 临床样本的糖谱检测与生物标志物发现;

    5. 与质谱技术联用研究糖基化蛋白质组。

     

     

     

    凝集素列表:

     

                    

                                                                                                                         

      动态箭头.gif详细列表下载

    蛋白芯片文献:


    1. Yang L, et al. Lectin Microarray Combined with Mass Spectrometry Identifies Haptoglobin-Related Protein (HPR) as a Potential Serologic Biomarker for Separating Nonbacterial Pneumonia from Bacterial Pneumonia in Childhood. Proteomics Clin Appl. 2018: e1800030. (上海儿童医学中心)


    2. Xin AJ, et al. Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility .Sci Rep-UK, 2016, 6(1): 20249.    (复旦大学生殖与发育研究院)


    3. Zhou S M, et al. Lectin RCA-I specifically binds to metastasis-associated cell surface glycans in triple-negative breast cancer. Breast Cancer Res, 2015, 17(1): 36.  (上海交通大学)


    4. Tan Z, et al. Altered N-glycan expression profile in epithelial-to-mesenchymal transition of NMuMG cells revealed by an integrated strategy using mass spectrometry and glycogene and lectin microarray analysis. J Proteome Res, 2014, 13(6): 2783-2795.


    5. Bird-Lieberman E L, et al. Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett's esophagus. Nat Med, 2012, 18(2): 315-321.


    6. Liu Y, et al. Identification and confirmation of biomarkers using an integrated platform for quantitative analysis of glycoproteins and their glycosylations. J Proteome Res, 2009, 9(2): 798-805.


    7. Tao S C, et al. Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers. Glycobiology, 2008, 18(10): 761-769.